RNA Silencing Data Package
Using RNA-seq, we provide a comprehensive analysis of the effect of RBP deregulation on gene expression patterns, expression levels and alternative splicing.
[bsf-info-box icon=”Defaults-flask” icon_size=”32″ icon_color=”#16b718″ title=”Bench work and results” pos=”left”]RT-PCR data for shRNA silencing efficiency in HeLa cells.[/bsf-info-box]
[bsf-info-box icon=”Defaults-cube” icon_size=”32″ icon_color=”#16b718″ title=”Basic Analysis” pos=”left”]Basic analysis of RNA-seq data, including sequencing quality assessment, clean reads assessment, mapping to human genome, sequencing saturation analysis and gene detection analysis, etc.[/bsf-info-box]
[bsf-info-box icon=”Defaults-cubes” icon_size=”32″ icon_color=”#16b718″ title=”Advanced Analysis” pos=”left”]Advanced analysis of RNA-seq data, including differentially expressed gene (DEG) analysis, alternative splicing event analysis and gene functional enrichment, etc.[/bsf-info-box]
[bsf-info-box icon=”Defaults-puzzle-piece” icon_size=”32″ icon_color=”#16b718″ title=”Data” pos=”left”]We provided the original RNA-seq data for two biological replicates and controls at 10G/sample.[/bsf-info-box]
Bonus! The package includes the shRNA silencing plasmid for each RBP.
- Provide shRNA target sequences.
- U6 polymerase III promoter for mammalian shRNA expression.
- Kanamycin and Blasticidin resistance markers for easy selection of transformed or transfected cells.
- MMLV LTR sequences for packaging into retroviral particles.
- pCMV-GFP expression to assess transfection efficiency.
- Suitable for creating transient or stable cell lines.