RBP Data Packages are the innovative products from ABLife Inc. (Appreciate the Beauty of Life), Wuhan, China. As we specialize in, we wish to share our years of research experience in the field of RNA biology and RBPs (RNA-binding proteins) with researchers around the globe. Through human RBP silencing and overexpression in HeLa cells, we provide cell biology and RNA-seq data for systematic studies of RBP functions and mechanisms!FIVE FEATURES WITH OUR RBP DATA PACKAGE PRODUCTS:
1. Originality: No similar products worldwide.
2. Quality: The quality of our data products meet the guidelines for publication in top scientific journals.
3. Uniqueness: Each RBP product is sold only once. Each data set you’ll receive is unique.
4. Comprehensiveness: From our line of products covering over 1,400 reported human RBPs, you’ll certainly find your gene of interest!
5. Timeliness: The complete products will be delivered on February 14, 2017. You don’t need to worry about the long waiting period and uncertainty of sequencing services.We believe that our products will be of great help and advancement to the scientific researchers within or wishing to break into RBP related fields. We sincerely hope that our best wishes can be delivered along with the product to the scientific researchers all over the world, including those in the “One Belt, One Road” countries.
We are offering a limited time and quantity presale at 25% off for the first 300 RBP data packages. For only RMB ￥37,000, you’ll be able to enjoy our RBP data package valued at RMB ￥49,800, which include:
RBP silencing (shRNA) or overexpression plasmids, for the silencing or overexpression of each of the RBPs in specific cell lines.
Cellular and molecular biology experimental data for the effects of RBP silencing or overexpression on cell proliferation, apoptosis and migration.
Transcriptomic (RNA-seq) data from RBP silencing/overexpression and control cell lines (2 biological replicates each).
Systematic analysis of RNA-seq data to facilitate your RBP function and mechanism studies.
Your choice of interested RBPs among our gene list.
Introduction
RNA binding proteins (RBPs) RNA is rarely at a loss for companions. Throughout their lifespan, RNAs are almost always accompanied by various RNA binding proteins (RBPs) to form ribonucleoprotein (RBP) complexes, which guide them through the journey of processing, nuclear export, localization and translation. Eukaryotic cells encode remarkably diverse RBPs, with highly specific recognition of their RNA targets, allowing delicate control of the generation, maturation, and lifespan of the RNA molecules.As key regulators of RNA metabolism, functional defects of RNA-binding proteins (RBPs) have profound implications for cellular physiology. According to the OMIM (Online Mendelian Inheritance in Man) database,mutations of over 150 RBPs have been shown to appear in human genetic diseases(1). RBP deregulations are associated with a number of neurodegenerative diseases and cancers. For example, mutations in the TARDBP/TPD-43 gene are commonly seen in Alzheimer’s disease (AD) and non-familial amyotrophic lateral sclerosis (ALS), and another RNA-binding protein FUS/TLS has also emerged as a significant disease protein in a subgroup of frontotemporal lobar dementias (FTLDs) and ALS(2–4). Additionally, a number of RNA-binding proteins are involved in the splicing deregulation of cancers(5, 6), which is becoming the new hot spot for developing targeted cancer therapy(7).
With over 1200 proteins in human being experimentally identified as harboring RNA binding functions(8–10), and hundreds more computationally predicted RBPs(11), ABLife is creating a brand-new line of products for studies on RBP functions and mechanisms, including a comprehensive data library of RBP-regulated transcriptome and RNA-protein interactome. With our RBP products, we hope to establish a new collaboration model that connects bioinformatics analysis and lab work, and provide new opportunities to facilitate and accelerate your research.
Reference:
- Castello,A., Fischer,B., Hentze,M.W. and Preiss,T. (2013) RNA-binding proteins in Mendelian disease. Trends Genet., 29, 318–327.
- Hanson,K.A., Kim,S.H. and Tibbetts,R.S. (2012) RNA-binding proteins in neurodegenerative disease: TDP-43 and beyond. Wiley Interdiscip. Rev. RNA, 3, 265–285.
- D’Alton,S., Altshuler,M. and Lewis,J. (2015) Studies of alternative isoforms provide insight into TDP-43 autoregulation and pathogenesis. RNA, 21, 1419–1432.
- Rogelj,B., Easton,L.E., Bogu,G.K., Stanton,L.W., Rot,G., Curk,T., Zupan,B., Sugimoto,Y., Modic,M., Haberman,N., et al. (2012) Widespread binding of FUS along nascent RNA regulates alternative splicing in the brain. Sci. Rep., 2, 1–10.
- Sveen, a, Kilpinen,S., Ruusulehto,A., Lothe,R. a and Skotheim,R.I. (2015) Aberrant RNA splicing in cancer; expression changes and driver mutations of splicing factor genes. Oncogene, 35, 1–15.
- Danan-Gotthold,M., Golan-Gerstl,R., Eisenberg,E., Meir,K., Karni,R. and Levanon,E.Y. (2015) Identification of recurrent regulated alternative splicing events across human solid tumors. Nucleic Acids Res., 43, 1–15.
- Chen,J. and Weiss,W. a (2015) Alternative splicing in cancer: implications for biology and therapy. Oncogene, 34, 1–14.
- Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M., Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M., et al. (2012) Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins. Cell, 149, 1393–1406.
- Kwon,S.C., Yi,H., Eichelbaum,K., Fohr,S., Fischer,B., You,K.T., Castello,A., Krijgsveld,J., Hentze,M.W. and Kim,V.N. (2013) The RNA-binding protein repertoire of embryonic stem cells. Nat. Struct. Mol. Biol., 20, 1122–1130.
- Baltz,A.G., Munschauer,M., Schwanh??usser,B., Vasile,A., Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K., Milek,M., et al. (2012) The mRNA-Bound Proteome and Its Global Occupancy Profile on Protein-Coding Transcripts. Mol. Cell, 46, 674–690.
- Sebestyén,E., Singh,B., Miñana,B., Pagès,A., Mateo,F., Pujana,M.A., Valcárcel,J. and Eyras,E. (2016) Large-scale analysis of genome and transcriptome alterations in multiple tumors unveils novel cancer-relevant splicing networks. Genome Res., 26, 732–744.